Progress in metabolic engineering of biosynthesis of 3-hydroxypropionic acid / 生物工程学报

As an important platform compound, 3-hydroxypropionic acid (3-HP) can be used as a substrate to synthesize a variety of biological products with commercial potential. The titer of 3-HP by wild-type bacteria is low, which severely limits the large-scale application and production of 3-HP. By modifying the genes related to the metabolic pathway, engineered bacteria using cheap substrates as carbon sources are constructed, the aim of reducing production cost and increasing output is realized. In this paper, the recent progress in the synthesis of 3-HP by metabolic engineering at home and abroad is reviewed. The advantages and disadvantages of glycerol pathway, malonyl-CoA pathway and beta-alanine pathway for synthesis of 3-HP are also summarized and analyzed, and the future development of 3-HP is prospected.

Effect of spousal voice stimulation on the recovery period of patients undergoing thoracic/lumbar surgery under general anesthesia / 中国实用护理杂志

Objective:To study the effect of spousal voice stimulation on the recovery period of patients undergoing thoracic/lumbar surgery under general anesthesia.Methods:A total of 67 patients undergoing thoracic/lumbar surgery under general anesthesia who met the inclusion exclusion criteria were divided into 34 patients in the control group and 33 patients in the experimental group according to the random number table method. Awakening in the recovery period patients were given general anesthesia care, on the basis of the control group recovery period voice wake up by paramedics. The recovery time, pain score, the incidence of restlessness and nausea and vital signs between the two groups were compared.Results:The extubation time, exhalation time, recovery time of choking cough, recovery time, and pain score in the recovery stage were (14.00±3.90) min, (11.67±3.83) min, (10.03±3.58) min, (21.24±4.28) min, (4.15±0.79) points in the experimental group, respectively. Those were (18.00±6.60) min, (15.59±5.86) min, (12.68±5.01) min, (29.50±8.25) min, (5.26±0.86) points in the control group, respectively. The differences between the two groups were statistically significant ( t values were 2.481-5.483, P<0.05 or 0.01). There was no significant difference in the incidence of restlessness and nausea and vital signs between the two groups( P>0.05). Conclusions:Spoused-voice stimulation can shorten the extubation time, exhalation time, recovery time and recovery time of choking cough in patients undergoing thoracolumbar surgery under general anesthesia, and reduce the pain in the recovery period.

Analyze the mediating effect of the positive psychological qualities, between emotional intelligence and humanistic caring ability among the nursing interns / 中国实用护理杂志

Objective@#To explore the humanistic caring ability, and to analyze the relationship between the positive psychological qualities, emotional intelligence and humanistic caring ability among the nursing interns, and provide theoretical basis to improve nursing students’ humanistic caring ability.@*Methods@#A total of 132 nursing interns from a three Level of first-class hospital in Liaoning province, were investigated by using the general questionnaire, caring ability inventory, emotional intelligence scale and Positive Mental Characters Scale for China normal university.@*Results@#The score of humanistic caring ability was (180.74±18.75). Among them, the average score of cognitive was the highest (73.71±10.93) and the average score of courage was the lowest (48.43±11.91). Emotional intelligence and positive psychological qualities were positively correlated with the humanistic caring ability, and positive psychological qualities was a intermediate variable between emotional intelligence and humanistic caring ability, and the mediating effect size is 18.71%.@*Conclusion@#The level of humanistic caring ability in nursing interns is lower, which needs to be further improved. The emotional intelligence and positive psychological qualities have a significant positive effect on the humanistic caring ability. Schools and internship hospitals can improve their sense of humanistic caring ability by developing the emotional intelligence and positive psychological qualities to stabilize the development of nursing career.

Analyze the mediating effect of the positive psychological qualities, between emotional intelligence and humanistic caring ability among the nursing interns / 中国实用护理杂志

Objective To explore the humanistic caring ability ,and to analyze the relationship between the positive psychological qualities , emotional intelligence and humanistic caring ability among the nursing interns, and provide theoretical basis to improve nursing students’humanistic caring ability. Methods A total of 132 nursing interns from a three Level of first-class hospital in Liaoning province, were investigated by using the general questionnaire, caring ability inventory, emotional intelligence scale and Positive Mental Characters Scale for China normal university. Results The score of humanistic caring ability was (180.74±18.75) . Among them, the average score of cognitive was the highest (73.71± 10.93) and the average score of courage was the lowest (48.43±11.91 ). Emotional intelligence and positive psychological qualities were positively correlated with the humanistic caring ability, and positive psychological qualities was a intermediate variable between emotional intelligence and humanistic caring ability, and the mediating effect size is 18.71%. Conclusion The level of humanistic caring ability in nursing interns is lower, which needs to be further improved. The emotional intelligence and positive psychological qualities have a significant positive effect on the humanistic caring ability. Schools and internship hospitals can improve their sense of humanistic caring ability by developing the emotional intelligence and positive psychological qualities to stabilize the development of nursing career.

Thoughts and practices of personnel training majoring in Chinese material medica based on the cultural self-confidence of Chinese medicine / 国际中医中药杂志

Guided by Chinese medicine culture,the strengthening of the cultural self-confidence of Chinese medicine is the cornerstone of the personnel training majoring in Chinese material medica.With the rapid development of modern medicine,the personnel training of the undergraduate students majoring in Chinese material medica in the universities of western medicine is facing several problems,such as lack of cultural identity,lack of professional confidence,unsteadiness of professional thinking,and changes of employment direction.For these problems,based on the main line "Solidify the cultural foundation of Chinese medicine,strengthen the self-confidence of Chinese material medica",our school selected the Chinese medicine cultural traveling,social practices and Chinese medicine employment guidance curriculum as the starting points,and carried out various of lively and vivid activities,which had positive impacts on the learning enthusiasm and professional love of the undergraduate students majoring in Chinese material medica.

A systematic review of biosynthesis of poly (3-hydroxypropionate) / 生物工程学报

Poly (3-hydroxypropionate) (P3HP), a new member of thermoplastic of family polyhydroxyalkanoates (PHAs), has excellent characteristics of biodegradability and biocompatibility. By now no reports can be found about wild-type bacteria that naturally synthesize P3HP, so the main way to produce P3HP is chemical and biological methods. Chemical method by adding high cost 3-HP monomers or their structural analogs as precursors, has the drawbacks of toxicity, low effectiveness and high cost. Biological method using engineered strain may utilize inexpensive and renewable carbon source to produce P3HP and has gradually become more and more popular. We systematically review here the biosynthesis of P3HP research progress. The advantages and disadvantages of biosynthesis pathways of glycerol pathway, malonyl-CoA pathway and β-alanine pathway were analyzed.

Design and practice of group PK teaching / 中华医学教育探索杂志

The author designed and practiced group PK teaching model in Pharmaceutical Botany combined with the ideas of educational skill training program in Peking university aiming at promoting students' learning initiative and enthusiasm as well as collective and teamwork sense.The paper discussed the concept of group PK teaching,requirements for teaching design and problems which should be noted in practice from the aspects of introduction,design and practice of the method as well as teaching effect evaluation and teaching method reflection.Survey questionnaire was used to evaluate the preliminary teaching effect of the new teaching method and to explore its teaching thought and educational idea.In practice,group PK teaching model improved students' self-learning awareness and ability,communication skills,collective and teamwork sense.

Analysis of broad-sense heritability and genetic correlation of production and content of glycyrrhizin of annual Glycyrrhiza uralensis / 中国中药杂志

<p><b>OBJECTIVE</b>To estimate the broad-sense heritability of the production of Glycyrrhiza uralensis and the content of glycyrrhizin as well as the genetic relationship of various growth indexes and biomass indexes, and provide the scientific basis for establishment of high quality licorice cultivate technology system.</p><p><b>METHOD</b>The randomized method was used to assign the provenance trial, the content of glycyrrhizin was determined by HPLC, and the method of classic genetics was applied to estimate the broad-sense heritability and genetic correlation coefficient.</p><p><b>RESULT AND CONCLUSION</b>The content of glycyrrhizin is influenced by the growth environment and gene, but the growth environment is the dominant factor. The estimated result of single sites about broad-sense heritability (h2) showed that the production of G. uralensis (W(u)) and the content of glycyrrhizin was controlled by gene which the broad-sense heritability was 0.663 2, 0.751 1 respectively, they had some potential on genetic modification. The results of genetic analysis correlation showed that the plant height and the stem diameter was positive (P < 0.01) correlated significantly with the production (W(u)) either on phenotype or on genetic, it suggests that the plant height and the stem diameter could be the index above ground to assessment the production of the G. uralensis. The content of glycyrrhizin had a positive correlation with the number of lateral root (P < 0.05), but it had a negative correlation with the plant height, stem diameter, diameter of root top (D(r)), the total biomass (W(t)) and the biomass underground (W(u)) on inheritance. It is suggested that it was difficult to achieve both high content and high yield simultaneously in the genetic improvement, so we should have a deeply thought about the specific improvement target when making the reformed scheme.</p>

Geographical variation of Glycyrrhiza uralensis seed germination character on water stress / 中国中药杂志

<p><b>OBJECTIVE</b>We studied the licorice provenance rule under the manual simulative water stress conditions, discussed geographical variation pattern and the ecology mechanism and laid the theoretical basis for the licorice seed regionalization and the seed allocation.</p><p><b>METHOD</b>On the spot we investigated and collected seed materials. Setting up water potential gradient by PEG, we observed the seed germination characteristic through the experiment of indoor germination.</p><p><b>RESULT</b>The sensitivity of various provenance seed germinative energy show quite remarkable difference for water potential reducing. Along with geographic longitude increasing, the seed germinates variation sensitivity increasing gradually to the water stress. The results of climatic factors' correlation analysis indicated that provenance seed germinate energy to the water stress is sensitive, in which the annual average temperature is low and annual precipitation is abundant. But it is insensitive in which the annual precipitation is little, the annual average temperature, average temperature in July and the annual average ground temperature is high.</p><p><b>CONCLUSION</b>Under the water stress condition the licorice provenance seed germination characteristic exists remarkable geographical variation. Geographical variation is the result of natural selection.</p>

Effect of Soil Quality on Lead and Copper Contents in the Tea / 环境与健康杂志

Objective In order to find the pollution source and provide some useful references for the controlling of the heavy metal pollution to the tea. Methods From March to April,2007,the soil and tea samples were collected from 10 tea gardens in the main producing areas. The soil samples were digested. The tea samples were dissolved by mix acid solution after being burnt into ashes,then the contents of lead and copper were determined by FAAS. Results There was a significant positive correlation between tea and soil in the lead contents (r lead=0.650 2,P

Anterior and posterior combined operation for treatment of lumbar tuberculosis / 中国矫形外科杂志

[Objective] To determine the efficacy of one-stage posterior deformity correction with transpedicular screw system and anterior radical debridement following reconstuction with titanium mesh cage for treatment of lumbar tuberculosis. [Methods]Forty-three patients with lumbar tuberculosis were treated operatively through anterior and posterior combined approach.The postoperative neurologic status (ASIA,2000),graft fusion,deformity correction and complications were observed and analyzed respectively.[Results]Forty-one patients were followed up for a duration of 24~36 months (mean 28.3 months).A solid fusion was achived in all cases.The neurologic function was improved in all patients in different grades.The mean kyphotic angle was corrected by 21.3 degrees,the angle loss was 1?~3?,the mean vertebral height was restored by 10.2 mm,the mean height loss was 2.4 mm.There was no recurrence of the tuberculosis infection.[Conclusion]One-stage posterior deformity correction with transpedicular screw system and anterior radical debridement following reconstuction with titanium mesh cage is a safe and effective method in the surgical management of lumbar tuberculosis.

Treatment of choledocholithiasis by biliary stent drainage with primary suture of the bile duct:A report of 16 cases / 中国微创外科杂志

Objective To summarize the experience of combination use of biliary stent placement and drainage with primary suture of the common bile duct in the treatment of choledocholithiasis. Methods Laparoscopic common bile duct exploration combined with flexible choledochoscopy was performed in 16 patients with choledocholithiasis from June 2005 to March 2006. During the operation, a patented “biliary stent for internal drainage” was introduced into the common bile duct, and primary sutures were performed to close the bile duct. Postoperatively, the stent was removed under gastroscope or duodenoscope. Results All the 16 operations were successfully accomplished without conversions to open surgery. The operative time was 90~260 min (mean, 145 min). The biliary stent was removed 5~7 days after the surgery. Follow-up for 3~12 months showed no residual stones or biliary stenosis. Conclusions Combination use of biliary stent drainage and primary suture of the common bile duct in the treatment of choledocholithiasis is safe and feasible.

Influence of drought stress on imbibition germination of Glycyrrhiza uralensis seed / 中草药

Object To study the influence of drought stress on imbibition germination of Glycyrrhiza uralensis seed and the drought resistance of its seedling. Methods To measure the imbibition rate, germination rate, seedling growth, relative tissue moisture content, relative electrical conductivity, SOD enzyme activity under drought stress simulated by PEG-6000. Results The imbibition rate, germination rate, SOD enzyme activity, and seedling growth present rising tendency then following by the decrease, while the relative tissue moisture content and the relative electrical conductivity tend to decrease. Conclusion The appropriate drought stress increases both the G. uralensis seed germination rate and the seedling drought resistance.

Expression Characteristics of Enzyme Activity of a Multi-functional Bacterial Community NSC-7 / 微生物学通报

In order to determine the degradation activity of the microbial community NSC-7, which had effi- cient cellulose and lindan degrading ability, degrading ability and four kinds of cellulase activity and hemi- cellulase activity were detected during degradation progress. The results showed that NSC-7 could degrade 73.6% of rice staw in weight, including 82.1% of cellulose, 58.2% of hemicellulase, and 5.4% of lignin within fourteen days. Endoglucanases, exoglucanases, ?-glucosidases and the total cellulase activities reached to maximum at the eighth day, and they were 4.48 U/mL, 15.83 U/mL, 25.78 U/mL and 7.51U /mL, respectively. The maximum of hemicellulase activity was 280.6 U/mL at the fifth day, and the average of hemicellulase activity was 43.71 times higher than cellulase activity.